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TBLASTX queries protein sequences to anucleotide sequence database with the sequences translated in all six reading frames.

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TBLASTX queries protein sequences to anucleotide sequence database with the sequences translated in all six reading frames.

(a) True

(b) False

I have been asked this question in an interview.

The origin of the question is Basic Local Alignment Search Tool (BLAST) topic in division Database Similarity Searching of Bioinformatics

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Right option is (b) False

The explanation: TBLASTN queries protein sequences to anucleotide sequence database with the sequences translated in all six reading frames. TBLASTX uses nucleotide sequences, which are translated in all six frames, to search against a nucleotide sequence database that has all the sequences translated in six frames. In addition, there is also a bl2seq program that performs local alignment of two user-provided input sequences. The graphical output includes horizontal bars and a diagonal in a two-dimensional diagram showing the overall extent of matching between the two sequences.

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