Question

Which of the following is an incorrect statement?

(a) SAGE and DNA microarrays are both high throughput techniques that determine global mRNA expression levels

(b) Studies have indicated that the gene expression measurements from these methods are highly inconsistent with each other

(c) SAGE does not require prior knowledge of the transcript sequence

(d) DNA microarray experiments can only detect the genes spotted on the microarray

This question was posed to me in a job interview.

I'm obligated to ask this question of Comparison of SAGE and DNA Microarrays in division Functional Genomics & Proteomics of Bioinformatics

Answer 1

Correct option is (b) Studies have indicated that the gene expression measurements from these methods are highly inconsistent with each other

Easiest explanation: SAGE has the potential to allow discovery of new, yet unknown gene transcripts. Because is able to measure all the mRNA expressed in a sample, it becomes possible.