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The PCR amplification step involved in the SAGE procedure means that it requires a large quantity of sample mRNA.

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The PCR amplification step involved in the SAGE procedure means that it requires a large quantity of sample mRNA.

(a) True

(b) False

I had been asked this question in class test.

I want to ask this question from Comparison of SAGE and DNA Microarrays topic in section Functional Genomics & Proteomics of Bioinformatics

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Best answer
Right option is (a) True

Easiest explanation: The PCR amplification step involved in the SAGE procedure means that it requires only a minute quantity of sample mRNA. This compares favorably to the requirement for a much larger quantity of mRNA for microarray experiments, which may be impossible to obtain under certain circumstances.

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