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DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.

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DNA microarrays measure “absolute” mRNA expression levels without arbitrary reference standards, whereas SAGE indicates the relative expression levels.

(a) True

(b) False

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I would like to ask this question from Comparison of SAGE and DNA Microarrays in division Functional Genomics & Proteomics of Bioinformatics

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Correct choice is (b) False

Best explanation: SAGE measures “absolute” mRNA expression levels without arbitrary reference standards, whereas DNA microarrays indicate the relative expression levels. Therefore, SAGE expression data are more comparable across experimental conditions and platforms. This makes public SAGE databases more informative by allowing comparison of data from reference conditions with various experimental treatments.

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