Question

The major challenges in genome assembly are sequence errors, contamination by bacterial vectors, and repetitive sequence regions.

(a) True

(b) False

The question was posed to me in my homework.

The doubt is from Genome Sequence Assembly in division Genome Mapping, Assembly and Comparison of Bioinformatics

Answer 1

Right answer is (a) True

To elaborate: Sequence errors can often be corrected by drawing a consensus from an alignment of multiple overlapped sequences. Bacterial vector sequences can be removed using filtering programs prior to assembly. To overcome the problem of sequence repeats, programs such as RepeatMasker can be used to detect and mask repeats. Additional constraints on the sequence reads can be applied to avoid miss-assembly caused by repeat sequences.