Question

The shotgun approach does not require knowledge of physical mapping of the clone fragments, but rather a robust computer assembly program to join the pieces of random fragments into a single, whole-genome sequence.

(a) True

(b) False

This question was addressed to me during an interview for a job.

This interesting question is from Genome Sequencing topic in chapter Genome Mapping, Assembly and Comparison of Bioinformatics

Answer 1

Correct option is (a) True

To explain: Generally, the genome has to be redundantly sequenced in such a way that the overall length of the fragments covers the entire genome multiple times. This is designed to minimize sequencing errors and ensure correct assembly of a contiguous sequence. Overlapping sequences with an overall length of six to ten times the genome size are normally obtained for this purpose.