Question

An alternative approach to determining protein–protein interactions is to use a large-scale affinity purification technique that involves attaching fusion tags to proteins and purifying the associated protein complexes in an affinity chromatography column.

(a) True

(b) False

The question was posed to me by my school principal while I was bunking the class.

I need to ask this question from Protein Interactions topic in section Functional Genomics & Proteomics of Bioinformatics

Answer 1

Right answer is (a) True

To explain I would say: The purified proteins are then analyzed by gel electrophoresis followed by MS for identification of the interacting components. The protein microarray systems mentioned above also provide a high throughput alternative for studying protein–protein interactions.