Question

Which of the following is incorrect about Data Collection?

(a) The two-color microarray uses multiple dyes at times

(b) The most common type of microarray protocol is the two-color microarray

(c) The cDNAs are obtained by extracting total RNA or mRNA from tissues or cells and incorporating fluorescent dyes in the DNA strands during the cDNA biosynthesis

(d) The expression of genes is measured via the signals from cDNAs hybridizing with the specific oligonucleotide probes on the microarray

I have been asked this question during an online interview.

I'd like to ask this question from Microarray-Based Approaches in division Functional Genomics & Proteomics of Bioinformatics

Answer 1

The correct option is (a) The two-color microarray uses multiple dyes at times

To explain: The most common type of microarray protocol is the two-color microarray, which involves labeling one set of cDNA from an experimental condition with one dye (Cy5, red fluorescence) and another set of cDNA from a reference condition (the controls) with another dye (Cy3, green fluorescence). When the two differently labeled cDNA samples are mixed in equal quantity and allowed to hybridize with the DNA probes on the chips, gene expression patterns of both samples can be measured simultaneously.