Question

In protein/domain analysis, each protein in the predicted proteome is again used as a query of a curated protein sequence database such as ____ in order to locate similar domains and sequences. To find orthologs, very low E value scores (E<10<20) for the alignment score and an alignment that includes 60–80% of the query sequence are generally required in order to avoid matches to paralogs.

(a) PubChem

(b) Genbank

(c) MeSH

(d) SwissProt

The question was asked during an online exam.

I would like to ask this question from Sequence Assembly and Gene Identification in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Correct answer is (d) SwissProt

To elaborate: The domain composition of each protein is also determined by searching for matches in domain databases such as Interpro. The analysis reveals how many domains and domain combinations are present in the proteome, and reveals any unusual representation that might have biological significance. The number of expressed genes in each family can also be compared to the number in other organisms to determine whether or not there has been an expansion of the family in the genome.