Question

Using cDNA sequence with the __________ it is much simpler to locate protein-encoding sequences in these molecules.

(a) exons taken out

(b) exons removed

(c) introns added

(d) introns removed

This question was addressed to me in class test.

Question is taken from Sequencing cDNA Libraries of Expressed Genes, Submission of Sequences to the Databases topic in portion Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Correct answer is (d) introns removed

To explain: The only possible difficulty is that a gene of interest may be developmentally expressed or regulated in such a way that the mRNA is not present. This problem has been circumvented by pooling mRNA preparations from tissues that express a large proportion of the genome, from a variety of tissues and developing organs or from organisms subjected to several environmental influences.