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Which of the following technique does not require the measurement of dissolved oxygen concentrations?

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Which of the following technique does not require the measurement of dissolved oxygen concentrations?

(a) Dynamic gassing out method

(b) Static gassing out method

(c) Oxygen-Balance method

(d) Sulphite oxidation method

The question was posed to me during an interview for a job.

My question comes from Measurement of KLa topic in portion Mass Transfer of Bioprocess Engineering

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The correct option is (d) Sulphite oxidation method

To explain: The oxygen-transfer rate is determined by the oxidation of sodium sulphite solution. This technique does not require the measurement of dissolved oxygen concentrations.

As oxygen enters solution it is immediately consumed in the oxidation of sulphite, so that the sulphite oxidation rate is equivalent to the oxygen-transfer rate. Since the dissolved oxygen concentration, is zero then the kLa may then be calculated from the equation:

kLa = OTR / C*

where OTR is the oxygen transfer rate.

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