Question

In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography?

(a) Western blotting

(b) Southern blotting

(c) Isopycnic centrifugation

(d) Spectrophotometry

I got this question in exam.

My query is from Nucleic-acid Techniques topic in section Techniques in Cell Biology of Cell Biology

Answer 1

Right choice is (b) Southern blotting

Best explanation: Southern blotting is the technique where nucleic acids are first transferred from gel to a nitrocellulose membrane. The membrane is then incubated in a solution of probes (complementary sequences) and then the bound sequences analyzed using autoradiography.