Question

Nucleic acid hybridization is used to identify ________________________

(a) RNAs

(b) DNAs

(c) Complementary base sequences

(d) Proteins

I have been asked this question in exam.

I'm obligated to ask this question of Nucleic-acid Techniques topic in division Techniques in Cell Biology of Cell Biology

Answer 1

Nucleic acid hybridization is a technique used to identify complementary base sequences in DNA or RNA molecules. This method involves pairing a labeled nucleic acid probe (either DNA or RNA) with its complementary sequence in the target nucleic acid. It allows for the detection and identification of specific sequences among a complex mixture of nucleic acids.

Here’s a breakdown of the options:

• (a) RNAs: While RNA can be detected, the technique specifically identifies complementary sequences rather than RNA alone.
• (b) DNAs: Similarly, DNA can be detected, but the goal is to find sequences that complement the probe.
• (c) Complementary base sequences: Correct. This is the primary purpose of nucleic acid hybridization.
• (d) Proteins: Proteins are not directly identified by this technique, as it is specific to nucleic acids.

Correct Answer: (c) Complementary base sequences