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In purification steps of nucleic-acids, phenol is used for _______________________

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In purification steps of nucleic-acids, phenol is used for _______________________

(a) deproteinization

(b) denaturation

(c) lowering pH

(d) lowering viscosity

I got this question by my school principal while I was bunking the class.

The origin of the question is Nucleic-acid Techniques topic in section Techniques in Cell Biology of Cell Biology

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The correct option is (a) deproteinization

Easiest explanation: After the release of nuclear content of a cell, the next step is to free the nucleic acids from proteins and RNA. Phenol is used to deproteinize the sample; a mixture of phenol and chloroform may also be used.

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