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Pre-replication complex is necessary to initiate replication. It assembles in______________

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Pre-replication complex is necessary to initiate replication. It assembles in______________

(a) Late Anaphase

(b) G1 phase

(c) S phase

(d) Early Anaphase

The question was posed to me in quiz.

My doubt stems from DNA Replication Eukaryotes topic in portion DNA Replication of Cytogenetics

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Correct answer is (b) G1 phase

Easy explanation: Pre-replication complex assembles on ORC in G1 phase. It is triggered in S phase when no new Pre-RC assembly takes place. Even during early Anaphase and late Anaphase this complex assembly is prevented.

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