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You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?

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You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?

(a) No growth

(b) One or two colonies

(c) Rapid growth with many colonies

(d) Normal growth with countable colonies

I have been asked this question in an interview.

This is a very interesting question from DNA Replication Eukaryotes in chapter DNA Replication of Cytogenetics

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The correct choice is (c) Rapid growth with many colonies

The explanation: ARS promotes replication of the yeast. Even if the plasmid was initially taken up by few cells due to rapid replication brought about by ARS the number of colony massively increases. If the same experiment was done with any random stretch of DNA none or few colonies would be seen.

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