Question

For performing EMSA what type of gel should be used?

(a) Native

(b) Denaturing SDS-PAGE

(c) Non-denaturing SDS PAGE

(d) Zymography

The question was asked in an interview for job.

This key question is from Transcription in Eukaryotes : Transcription Factors in portion Gene Expression : Transcription Prokaryotes of Cytogenetics

Answer 1

The correct choice is (c) Non-denaturing SDS PAGE

To elaborate: A denaturing SDS PAGE would break the interactions between the factors which is being studied. Thus, a non-denaturing SDS PAGE is used. A native page on the other hand would be less clear while zymography is unrelated.