Question

You take a crude cell lysate and treat it with DNase. Now wash it and add protease. Use specific restriction endonuclease to get your desired fragment and run it on a gel. You see a single band. Which of the following will be true about the fragment?

(a) It is actively transcribed

(b) Only part of it is transcribed

(c) No part of it transcribed

(d) Undefined transcriptional nature

I have been asked this question in an interview for internship.

I'd like to ask this question from Transcription in Eukaryotes : Chromatin Remodelling in portion Gene Expression : Transcription Prokaryotes of Cytogenetics

Answer 1

Correct option is (c) No part of it transcribed

Explanation: DNase would only get access to uncondensed DNA. Uncondensed DNA is transcriptionally active. As the DNase would have cleaved the fully or partially transcriptionally active DNA, and our fragment is intact, we can say it was not at all being transcribed.