Question

The eukaryotic initiation codon recognizes________

(a) f-Met-tRNA-f-Met

(b) Met-tRNAi-Met

(c) f-Met-tRNAi-Met

(d) f-Met-tRNA-Met

I have been asked this question in an interview for internship.

My enquiry is from Translation in Eukaryotes topic in division Gene Expression : Translation of Cytogenetics

Answer 1

The correct option is (c) f-Met-tRNAi-Met

To explain I would say: The initiation of translation in eukaryotes is by the codon AUG that recognizes initiation tRNA or tRNAi that binds to methionine. While in prokaryotes it is by formyl met tRNA which binds to met and converts it to formyl met.