Question

Choose the endonuclease that can cuts double strand of DNA.

(a) Mut H

(b) Ruv C

(c) EcoRI

(d) S1

I had been asked this question by my college professor while I was bunking the class.

My doubt stems from Repair of DNA Damage: Recombinational and Bypass topic in section DNA Mutation, DNA Repair, Transposable Elements of Cytogenetics

Answer 1

Right answer is (c) EcoRI

Easy explanation: ECoRI is a restriction endonuclease that can cut both the DNA strands generating an overhand. On the other hand Ruv C in recombination repair, Mut H in MMR and S1 endonuclease can only make single strand nicks.