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What is the stretch of free DNA needed by NER machinery to repair the bulky lesions in TC NER?

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in Chemical Reaction Engineering by (679k points)
What is the stretch of free DNA needed by NER machinery to repair the bulky lesions in TC NER?

(a) 50 nt

(b) 100 nt

(c) 200 nt

(d) 250 nt

I had been asked this question in exam.

My query is from Repair of DNA Damage: NER, Direct Reversal in section DNA Mutation, DNA Repair, Transposable Elements of Cytogenetics

1 Answer

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The correct answer is (a) 50 nt

Easiest explanation: A stretch of free 100 nt is needed by NER machinery to excise out and replace the lesion. Thus, either the transcribing RNA polymerase then has to backtrack or it falls off.

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