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What does this sequence GC/GGCCGC recognized and cleaves by Not1 Doesn’t agree to?

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What does this sequence GC/GGCCGC recognized and cleaves by Not1 Doesn’t agree to?

(a) The cleavage is after the 2^nd C from 5’ end

(b) The cleavage generates a 5’ overhang

(c) This sequence has a probability of occurrence in every 1024 bases

(d) This cleavage is asymmetric

The question was posed to me by my college professor while I was bunking the class.

The above asked question is from Restriction Endonuclease in chapter Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

1 Answer

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The correct answer is (c) This sequence has a probability of occurrence in every 1024 bases

The explanation: The probability of occurrence of an 8 bp recognition sequence can be determined as 4^8, this is because there are 4 bases and 8 specific bases in position in this sequence; which is equal to 65536. However the rest of the options are true.

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