Correct answer is (b) Physically breaking the cells
The best explanation: Bead transfection combines the principle of physically producing breaks in the cellular membrane using beads. In this method, the adherent cells are incubated for a brief period with glass beads in a solution containing the DNA. Suspending cells in electroporation cuvette is for electroporation. Biolistic is also known as particle bombardment. Ultrasound is used in sonoporation.