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Bead transfection is physically producing breaks in the cellular membrane using _____________

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Bead transfection is physically producing breaks in the cellular membrane using _____________

(a) Beads

(b) Electric current

(c) Ultrasound

(d) Laser light

I had been asked this question at a job interview.

This intriguing question originated from Genetic Recombination in chapter Genetic Recombination of Drug Biotechnology

1 Answer

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Best answer
Correct answer is (a) Beads

Explanation: Bead transfection produces breaks in the cellular membrane using beads. The adherent cells, glass beads and solution containing the DNA are all incubated together. Then the solution is mixed such that the beads collide with each other so that any cells coming in between two beads will get a mechanical shock producing small pores.

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