Question

How inoculum is prepared in the production of antibiotics?

(a) On solid media

(b) On liquid media

(c) First on solid media than on liquid media

(d) On suspension

This question was addressed to me in exam.

Enquiry is from Production of Antibiotics (Isolation of Fermentation Products) topic in division Antibiotics & Enzyme Immobilization of Drug Biotechnology

Answer 1

The correct option is (c) First on solid media than on liquid media

To elaborate: Inoculum development begins on solid media, and then liquid media are used; the media used are specific for inoculum development. Spore suspension is prepared for inoculum. This will be then transferred to the fermenter.