Question

Proteolytic enzyme trypsin cleaves protein at which of these specific sites?

(a) Carboxyl side of Alanine, Glycine

(b) Carboxyl side of Lysin or Arginine

(c) Carboxyl side of aromatic amino acid

(d) The amino side of aromatic amino acid

I have been asked this question during an interview for a job.

Asked question is from Protein Folding in portion Biomolecules and Catalysis of Life Sciences

Answer 1

Right choice is (b) Carboxyl side of Lysin or Arginine

To elaborate: The specificity of these enzymes determined by the side chain of amino acid on either side of a peptide bond. This cleavage is necessary to sequence protein which is made up of more than 50 amino acid residues.