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Which one of the following reagent is also known a Sanger’s reagent?

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Which one of the following reagent is also known a Sanger’s reagent?

(a) 1-fluoro-2,4-dinitrobenzene (FDNB)

(b) Phenylisothiocyanate

(c) Cyanogen bromide

(d) β-mercaptoethanol

I have been asked this question in a dream while sleeping

This is a very interesting question from Protein Folding topic in division Biomolecules and Catalysis of Life Sciences

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Correct choice is (a) 1-fluoro-2,4-dinitrobenzene (FDNB)

The best explanation: FDNB reacts with a free amino group of N-terminal amino acid in alkaline solution to form yellow dinitrophenol. This reaction was first used by Sanger to determine the primary structure of the polypeptide hormone.

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