Question

Which of the following is untrue regarding PSIPRED?

(a) It is a web-based program that predicts protein secondary structures

(b) It uses a combination of evolutionary information and neural networks

(c) It uses a combination of evolutionary information only

(d) The multiple sequence alignment is derived from a PSI-BLAST database search

I have been asked this question in an online interview.

Question is from Coiled Coil Prediction in division Secondary Structure Prediction of Bioinformatics

Answer 1

Correct choice is (c) It uses a combination of evolutionary information only

To elaborate: A profile is extracted from the multiple sequence alignment generated from three rounds of automated PSI-BLAST. The profile is then used as input for a neural network prediction similar to that in PHD, but without the jury layer. To achieve higher accuracy, a unique filtering algorithm is implemented to filter out unrelated PSI-BLAST hits during profile construction.