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Most vertebrate genes use __________ as the translation start codon and have a uniquely conserved flanking sequence call a Kozak sequence (CCGCCATGG).

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Most vertebrate genes use __________ as the translation start codon and have a uniquely conserved flanking sequence call a Kozak sequence (CCGCCATGG).

(a) AAG

(b) ATG

(c) AUG

(d) AGG

The question was posed to me in an interview.

I want to ask this question from Gene Prediction in Eukaryotes topic in portion Gene and Promoter Prediction of Bioinformatics

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The correct choice is (b) ATG

To explain: In addition, most of these genes have a high density of CG dinucleotides near the transcription start site. This region is referred to as a CpG island (p refers to the phosphodiester bond connecting the two nucleotides), which helps to identify the transcription initiation site of a eukaryotic gene. The poly-A signal can also help locate the final coding sequence.

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