Question

Which of the following is incorrect regarding 2D-Page?

(a) Not all proteins can be separated by this method or stained properly

(b) The stained gel can be scanned and digitized for image analysis

(c) Membrane proteins are largely hydrophilic and readily solubilized

(d) One of the challenges of this technique is the separation of membrane proteins

I have been asked this question during an online interview.

Query is from Technology of Protein Expression Analysis topic in chapter Functional Genomics & Proteomics of Bioinformatics

Answer 1

Correct option is (c) Membrane proteins are largely hydrophilic and readily solubilized

Easy explanation: Membrane proteins are largely hydrophobic and not readily solublized. They tend to aggregate in the aqueous medium of a two-dimensional gel. To overcome this problem, membrane proteins can be fractionated using specialized protocols and then electrophoresed using optimized buffers containing zwitterionic detergents. Subfractionation can be carried out to separate nuclear, cytosol, cytoskeletal, and other subcellular fractions to boost the concentrations of rare proteins and to reveal subcellular localizations of the proteins.