Question

Which of the following is incorrect regarding Differential In-Gel Electrophoresis?

(a) Proteins are mixed together before electrophoresis on a two-dimensional gel

(b) Differentially expressed proteins in both conditions can’t be visualized in the same gel

(c) In this, Differences in protein expression patterns can be detected in a similar way as in fluorescent-labeled DNA microarrays

(d) Proteins from experimental and control samples are labeled with differently colored fluorescent dyes

The question was asked in an online quiz.

I need to ask this question from Technology of Protein Expression Analysis topic in portion Functional Genomics & Proteomics of Bioinformatics

Answer 1

Right option is (b) Differentially expressed proteins in both conditions can’t be visualized in the same gel

For explanation I would say: Differentially expressed proteins in both conditions can be co-separated and visualized in the same gel. Compared to regular 2D-PAGE, the process reduces the noise and improves the reproducibility and sensitivity of detection. In principle, it resembles the two-color DNA microarray analysis. The drawbacks of this approach are that different proteins take up fluorescent tags to different extents and that some proteins labeled with the fluorophores may become less soluble and precipitate before electrophoresis.