Question

The shotgun approach _______ sequences clones from _____ of cloned DNA.

(a) randomly, one end

(b) randomly, both ends

(c) specifically, both ends

(d) specifically, one end

This question was addressed to me by my college director while I was bunking the class.

This question is from Genome Sequencing topic in chapter Genome Mapping, Assembly and Comparison of Bioinformatics

Answer 1

Correct option is (b) randomly, both ends

For explanation I would say: There are two major strategies for whole genome sequencing: the shotgun approach and the hierarchical approach. The shotgun approach generates a large number of sequenced DNA fragments. The number of random fragments has to be very large, so large that the DNA fragments overlap sufficiently to cover the entire genome.