Question

In hierarchical genome sequencing approach, based on the results of _______ mapping _______of the BAC clones on a chromosome can be determined.

(a) physical, the locations and orders

(b) physical, only the locations

(c) cytological, only the locations

(d) physical, only the orders

The question was posed to me by my college professor while I was bunking the class.

This intriguing question originated from Genome Sequencing topic in section Genome Mapping, Assembly and Comparison of Bioinformatics

Answer 1

Right option is (a) physical, the locations and orders

Easiest explanation: By successively sequencing adjacent BACclone fragments, the entire genome can be covered. The complete sequence of each individual BAC clone can be obtained using the shotgun approach. Overlapping BAC clones are subsequently assembled into an entire genome sequence.