Question

Which of the following is untrue regarding EST Index Construction?

(a) The goal of the EST databases is to organize and consolidate the largely redundant EST data

(b) The process includes a preprocessing step that removes masks repeats

(c) There is no screening of vector contaminants

(d) The goal of the EST databases is to improve the quality of the sequence information so the data can be used to extract full-length cDNAs

The question was posed to me during an interview.

I want to ask this question from Sequence in portion Functional Genomics & Proteomics of Bioinformatics

Answer 1

The correct answer is (c) There is no screening of vector contaminants

The explanation: The process includes a preprocessing step that removes vector contaminants and masks repeats. Vecscreen, can be used to screen out bacterial vector sequences. This is followed by a clustering step that associates EST sequences with unique genes.