Question

Screening the predicted protein sequences against ______ library confirms the prediction and expression of the gene.

(a) expressed sequence tag (EST)

(b) tags

(c) palindromes

(d) proteomes

I got this question in semester exam.

My doubt stems from Sequence Assembly and Gene Identification topic in portion Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

The correct answer is:

(a) expressed sequence tag (EST)

Expressed Sequence Tags (ESTs) are short sequences derived from the transcript of genes that are expressed in a given tissue or condition. Screening predicted protein sequences against an EST library helps confirm the prediction and expression of the gene, as ESTs correspond to sequences of mRNA and provide evidence that a gene is being actively transcribed and translated. This helps validate the gene prediction in the genome assembly.

So, (a) EST is the most appropriate answer.