Question

In Proteolysis and fragment sequencing, Protein spots may be excised from a two-dimensional protein gel and subjected to a combination of amino acid sequencing and cleavage analyses using the techniques of mass spectrometry and high-pressure liquid chromatography.

(a) True

(b) False

I have been asked this question during an interview.

My doubt is from Sequence Assembly and Gene Identification topic in division Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

The correct answer is (a) True

Easy explanation: Genome regions that encode these sequences can then be identified and the corresponding gene located. A similar method may be used to identify the gene that encodes a particular protein that has been purified and characterized in the laboratory.