Question

In Protein 2D gel Electrophoresis, Individual proteins produced by the genome can be separated to _____ by this method and specific ones identified by various _______

(a) smaller extent, biochemical and immunological tests

(b) a large extent, biochemical and immunological tests

(c) a large extent, biochemical tests only

(d) smaller extent, purely mechanical tests

I have been asked this question in an interview.

I'm obligated to ask this question of Sequence Assembly and Gene Identification topic in section Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Correct choice is (b) a large extent, biochemical and immunological tests

Easy explanation: Moreover, changes in levels of proteins in response to an environment signal can be monitored in much the same way as a microarray analysis is performed. Microarrays only detect untranslated mRNAs, whereas a two-dimensional gel protein analysis detects translation products, thus revealing an additional level of regulation.