Question

Which of the given statement is incorrect about proteome analyses?

(a) For BLAST, setting an effective database size appropriate for each search and program is important

(b) Due to the large number of comparisons that must be made in these types of analyses and due to the volume of program output, the procedure must be automated on a local machine using Perl scripts or a similar method and a database system

(c) BLAST is used for obtaining a correct statistical evaluation of alignment scores

(d) BLAST does not give statistical evaluation of alignment scores

I got this question in examination.

This interesting question is from Sequence Assembly and Gene Identification topic in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Right option is (d) BLAST does not give statistical evaluation of alignment scores

The best I can explain: Each protein encoded by the genome is used as a query in database similarity searches to identify similar database proteins, some having a known structure or function. Additional searches of EST databases can be used to identify additional relatives of the query sequence.