Question

In ion-exchange chromatography, proteins bound to the resin can be displaced by increasing the _____________________

(a) strength of ionic buffer

(b) size of sample

(c) column volume

(d) column width

This question was addressed to me in class test.

I would like to ask this question from Protein Techniques in chapter Techniques in Cell Biology of Cell Biology

Answer 1

The correct option is (a) strength of ionic buffer

For explanation: In ion-exchange chromatography, the proteins bind reversibly to the inert resin through ionic bonds. To displace and eventually elute the bound proteins the ionic strength of buffer can be increased.