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Polyacrylamide gel electrophoresis uses ___________________ to separate proteins.

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Polyacrylamide gel electrophoresis uses ___________________ to separate proteins.

(a) pressure difference

(b) temperature difference

(c) electric field

(d) magnetic field

This question was posed to me during an interview.

This intriguing question comes from Protein Techniques topic in chapter Techniques in Cell Biology of Cell Biology

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Correct answer is (c) electric field

Explanation: Polyacrylamide gel electrophoresis (PAGE) is a powerful technique that uses electric field to separate out proteins in a matrix. Small organic molecules of acrylamide are cross-linked to form a molecular sieve through which the proteins travel.

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