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Proteins interacting with specific substances can be separated using ___________________

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Proteins interacting with specific substances can be separated using ___________________

(a) ion-exchange chromatography

(b) paper chromatography

(c) affinity chromatography

(d) gel-filtration chromatography

The question was asked in a national level competition.

This intriguing question originated from Protein Techniques in section Techniques in Cell Biology of Cell Biology

1 Answer

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Best answer
Correct option is (c) affinity chromatography

Best explanation: The proteins participating in interactions such as ligand-ligand, enzyme-substrate etc. can be purified using the affinity chromatography. In this technique, an interacting molecule is covalently attached to the inert matrix.

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