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Gel-filtration chromatography separates proteins based on their ___________________

(a) pH

(b) temperature

(c) morphology

(d) effective size

The question was posed to me in an internship interview.

I want to ask this question from Protein Techniques in division Techniques in Cell Biology of Cell Biology

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Right answer is (d) effective size

To explain: Gel-filtration chromatography separates the proteins based on their effective size (hydrodynamic radius). The column material consists of cross-linked polysaccharides like agarose and dextrans.

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