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Which technique can be used to separate ^15N and ^14N?

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Which technique can be used to separate ^15N and ^14N?

(a) Rate-zonal centrifugation

(b) Isopycnic centrifugation

(c) HPLC

(d) Mass spectrometry

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This question is from Nucleic-acid Techniques in section Techniques in Cell Biology of Cell Biology

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The correct option is (b) Isopycnic centrifugation

Easy explanation: Isopycnic or equilibrium centrifugation can be used to separate nucleic acids having different base composition and even different isotopes of nitrogen such as ^15N and ^14N.

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