Question

When the pH of a protein is lowered _________________________

(a) temperature decreases

(b) negatively-charged groups neutralize

(c) positively-charged groups neutralize

(d) positively-charged groups decrease

The question was posed to me in an online interview.

My doubt is from Protein Techniques topic in portion Techniques in Cell Biology of Cell Biology

Answer 1

Right choice is (b) negatively-charged groups neutralize

To explain: When the pH (potential hydrogen) of a protein is lowered, the negatively charged groups become neutralized whereas the positively-charged groups become abundant. The opposite occurs when the pH increases.