Question

AP endonuclease can cleave the DNA at__________ while AP lyase will cleave the DNA at__________

(a) 5’, 3’

(b) 3’, 3’

(c) 3’, 5’

(d) 5’, 5’

I had been asked this question in an interview.

I'd like to ask this question from Repair of DNA Damage: MMR, BER in section DNA Mutation, DNA Repair, Transposable Elements of Cytogenetics

Answer 1

Right answer is (a) 5’, 3’

The best explanation: The AP endonuclease can cleave at the 5’ end of the Apurinic or Apyrimidine site, while the bifunctional AP lyase cleaves at the 3’ end.