Question

Despite the multiple coverage, sometimes certain genomic regions remain unsequenced, mainly owing to cloning difficulties.

(a) True

(b) False

I have been asked this question in class test.

The query is from Genome Sequencing topic in portion Genome Mapping, Assembly and Comparison of Bioinformatics

Answer 1

Right option is (a) True

Easy explanation: In such mentioned cases, the remainder gap sequences can be obtained through extending sequences from regions of known genomic sequences using a more traditional PCR technique. That which requires the use of custom primers and performs genome walking in a stepwise fashion. This step of genome sequencing is also known as finishing, which is followed by computational assembly of all the sequence data into a final complete genome.