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The justification behind Rosetta stone method is that when two domains are fused in a single protein, they have to be in _______ proximity to perform a common function.

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The justification behind Rosetta stone method is that when two domains are fused in a single protein, they have to be in _______ proximity to perform a common function.

(a) distant

(b) close

(c) extremely distant

(d) extremely close

I have been asked this question during an online interview.

Enquiry is from Protein Interactions topic in portion Functional Genomics & Proteomics of Bioinformatics

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The correct answer is (d) extremely close

The explanation is: When the two domains are located in two different proteins, to preserve the same functionality, their close proximity and interaction have to be preserved as well. Therefore, by studying gene/protein fusion events, protein–protein interactions can be predicted.

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