Question

The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.

(a) True

(b) False

This question was addressed to me in examination.

Origin of the question is DNA & Genomic Sequencing in chapter Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Correct choice is (a) True

Best explanation: For accurate work, a printout of the scan is usually examined for abnormalities that decrease the quality of the sequence, and the sequence may then be edited manually. The first sequence can also be extended by making a second oligonucleotide matching the distal end of the readable sequence and using this primer to read more of the original template.