Question

An alternative method is to sequence all the subclones, produce a computer database of the sequences, and then have the computer assemble the sequences from the overlaps that are found.

(a) True

(b) False

This question was posed to me in unit test.

This interesting question is from DNA & Genomic Sequencing topic in portion Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Right answer is (a) True

Best explanation: Up to 10 levels of redundancy are used to get around the problem of a small fraction of abnormal clones. This procedure was first used to obtain the sequence of the 4- Mb chromosome of the bacterium Haemophilus influenzae by The Institute of Genetics Research (TIGR) team. Only a few regions could not be joined because of a problem subcloning those regions into plasmids, requiring manual sequencing of these regions from another library of phage subclones.