Question

Which of the following is untrue about Shotgun Sequencing?

(a) When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to overlap

(b) When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to scrutinize

(c) In a new whole shotgun approach, Celera Genomics is sequencing both ends of DNA fragments of short (2 kb), medium (10 kb), and long (BAC or >100 kb) lengths

(d) A large number of reads are then assembled by computer

This question was addressed to me by my college director while I was bunking the class.

Query is from DNA & Genomic Sequencing topic in portion Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Correct answer is (b) When DNA fragments derived from different chromosomal regions have repeats of the same sequence, they will appear to scrutinize

The explanation: A controversy has arisen as to whether or not the above shotgun sequencing strategy can be applied to genomes with repetitive sequences such as those likely to be encountered in sequencing the human genome. This method has been used to assemble the genome of the fruit fly Drosophila melanogaster after removal of the most highly repetitive regions and also to assemble a significant proportion of the human genome.